Objectives

The present study focuses on in-vitro and in-silico investigation of the root extract of Premna integrifolia Linn. (Agnimantha), a plant widely used in traditional indian medicine for treating various ailments such as inflammation, immune disorders, gastrointestinal issues, and skin conditions.

Methodology

The phytochemical analysis and evaluation of anti-inflammatory and antioxidant properties were carried out using the COX-2 inhibition assay (ELISA kit) and DPPH radical scavenging assay respectively.

Results

The root extract exhibited significant anti-inflammatory activity, with an IC50 value demonstrating dose-dependent inhibition when compared to Mefenamic acid as the standard. The DPPH assay showed notable antioxidant activity, indicated by the colour change from purple to yellow, reflecting free radical scavenging. Additionally, the in-silico analysis, performed using Schrödinger’s Glide software, revealed a strong binding affinity of the phytoconstituents with selected target proteins [PDB ID: 5G3M], [PDB ID: 4XTA], and [PDB ID: 3U8D], further supporting the therapeutic potential of Premna integrifolia in anti-inflammatory treatment.

Conclusion

This study shows that Premna integrifolia Linn. has considerable anti-inflammatory and antioxidant properties, which are validated by both experimental and computational results. The favourable phytochemical profile and molecular docking results indicate that this plant could be a potential source for creating natural therapeutic agents that target inflammation and oxidative stress-related disorders.